The most part of DeskTool settings is kept in desktool.cfg file (by default c:\Program Files\DeskTool\DeskTool.cfg). It is enough to backup this file to save DeskTool objects` structure or to replace this file, if necessary.
However one of these must be done. The configuration file (desktool.cfg) should be placed in the same folder as DeskTool.exe.
The registry keys will be recreated at next run of DeskTool (except registration recording).